Fig 1: Effect of FIGNL1 knockdown on in vivo tumor formation. BALB/c nude mice were divided into two groups: One group were injected with shCtrl H1299 cells, while the other group were injected with shFIGNL1-2 H1299 cells. The statistical graph indicated that compared with that in the mice injected with shCtrl, FIGNL1 knockdown significantly reduced the formation of the tumor from day 13. ***P<0.001, ****P<0.0001. n=7. sh, short hairpin; Ctrl, control; FIGNL1, fidgetin-like 1.
Fig 2: Representative immunohistochemical images showing FIGNL1 expression in cancerous and para-carcinoma tissues. (A) FIGNL1 negative staining in para-carcinoma tissues (score, 0). (B) Negative FIGNL1 staining in carcinoma tissues (score, 0). (C) Weak positive staining for FIGNL1 in cancerous tissues (score, 1). (D) Moderate positive staining for FIGNL1 in cancerous tissues (score, 2). (E) Strong positive staining for FIGNL1 in cancerous tissues (score, 3). FIGNL1, fidgetin-like 1. SP, streptavidin-peroxidase.
Fig 3: RT-qPCR and western blot analysis of mRNA and protein expression level for the genes identified from the microarray results. (A) RT-q PCR quantification and (B) western blot grayscale image and expression analysis of the selected downregulated genes. **P<0.01, ***P<0.001, ****P<0.0001. n=3. sh, short hairpin; Ctrl, control, RT-qPCR, reverse transcription-quantitative PCR; PCNA, proliferating cell nuclear antigen; MCM, mini-chromosome maintenance complex component, SKP2, S-phase kinase associated protein 2; FIGNL1, fidgetin-like 1.
Fig 4: FIGNL1 protein and mRNA expression level in H460, H23, H1299 and A549 lung cancer cells determined using (A) reverse transcription-quantitative PCR and (B) western blot analysis. FIGNL1, fidgetin-like 1.
Fig 5: Effects of FIGNL1 knockdown and overexpression on wound healing ability in the H1299 cell line. Knockdown of FIGNL1 significantly reduced migration ability of the cells, while FIGNL1 overexpression increase migration compared with that in the respective negative control groups. *,#P<0.05, **P<0.01. n=3. NC, negative control; OE, overexpression; sh, short hairpin; Ctrl, control; FIGNL1, fidgetin-like 1.
Supplier Page from Abcam for Anti-FIGNL1 antibody